ROCK2 Antikörper (AA 652-923)

Produktnummer ABIN3043921

Produktdetails anti-ROCK2 Antikörper

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Bindungsspezifität: AA 652-923

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ROCK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Rho-associated, coiled-coil containing protein kinase 2
Protein Name: Rho-associated protein kinase 2

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ROCK2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-ROCK2 Antikörper (ABIN3043921)

Qiao, Zou, Liu, Zhu, Wang, Yang, Jiang, Chen: "ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension." in: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2567-2572, (2016) (PubMed).

Yan, Wang, Jiang, Chen, He, Mang, Shao, Xu: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." in: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).

Wang, Wang, Liu, Bai, Zhang, Sun, Wang: "Fluoxetine inhibits monocrotaline-induced pulmonary arterial remodeling involved in inhibition of RhoA-Rho kinase and Akt signalling pathways in rats." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 11, pp. 1506-15, (2012) (PubMed).

Ma, Zhang, Li, Liu, Ma, Li, Falck, Manthati, Reddy, Medhora, Jacobs, Zhu: "8,9-Epoxyeicosatrienoic acid analog protects pulmonary artery smooth muscle cells from apoptosis via ROCK pathway." in: Experimental cell research, Vol. 316, Issue 14, pp. 2340-53, (2010) (PubMed).

Details zu ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Andere Bezeichnung: ROCK2 (ROCK2 Produkte)

Hintergrund: Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

Synonyms: coiled-coil-containing protein kinase 2 antibody|KIAA0619 antibody|p164 ROCK 2 antibody|p164 ROCK-2 antibody|Rho associated coiled coil containing protein kinase 2 antibody|Rho associated protein kinase 2 antibody|Rho associated, coiled coil containing protein kinase II antibody|Rho kinase 2 antibody|Rho-associated antibody|Rho-associated protein kinase 2 antibody|ROCK 2 antibody|Rock II antibody|Rock2 antibody|ROCK2_HUMAN antibody|Rock2m antibody|ROK alpha antibody|ROKalpha antibody

Gen-ID: 9475

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signalweg, Tube Formation