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HSD11B2 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSD11B2 in WB, IHC, IHC (fro) und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3043846

Kurzübersicht für HSD11B2 Antikörper (C-Term) (ABIN3043846)

Target

Alle HSD11B2 Antikörper anzeigen
HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Reaktivität

  • 44
  • 20
  • 10
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 61
  • 6
Kaninchen

Klonalität

  • 63
  • 4
Polyklonal

Konjugat

  • 35
  • 4
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser HSD11B2 Antikörper ist unkonjugiert

Applikation

  • 53
  • 23
  • 23
  • 14
  • 14
  • 11
  • 10
  • 9
  • 5
  • 4
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
  • Bindungsspezifität

    • 16
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 277-309, C-Term

    Verwendungszweck

    Anti-HSD11B2 Antibody Picoband®

    Sequenz

    EKRKQLLLAN LPQELLQAYG KDYIEHLHGQ FLH

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-HSD11B2 Antibody (ABIN3043846). Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human HSD11B2, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry(Frozen Section), 2-5 μg/mL, Human, Mouse, Rat
    Immunofluorescence, 5 μg/mL, Human
    1. Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS (November 1994). "Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme". Mol. Cell. Endocrinol. 105 (2): R11-7. 2. Brown RW, Chapman KE, Kotelevtsev Y, Yau JL, Lindsay RS, Brett L, Leckie C, Murad P, Lyons V, Mullins JJ, Edwards CR, Seckl JR (February 1996). "Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2". Biochem. J. 313 ( Pt 3) (Pt 3): 1007-17.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

    Andere Bezeichnung

    HSD11B2

    Hintergrund

    Synonyms: Corticosteroid 11-beta-dehydrogenase isozyme 2,1.1.1.-,11-beta-hydroxysteroid dehydrogenase type 2,11-DH2,11-beta-HSD2,11-beta-hydroxysteroid dehydrogenase type II,11-HSD type II,11-beta-HSD type II,NAD-dependent 11-beta-hydroxysteroid dehydrogenase,11-beta-HSD,Short chain dehydrogenase/reductase family 9C member 3,HSD11B2,HSD11K , SDR9C3,

    Tissue Specificity: Expressed in kidney, pancreas, prostate, ovary, small intestine and colon. At midgestation, expressed at high levels in placenta and in fetal kidney and, at much lower levels, in fetal lung and testis (PubMed:8530071). .

    Background: Corticosteroid 11-β-dehydrogenase isozyme 2, also known as?11-β-hydroxysteroid dehydrogenase 2,?is an enzyme?that in humans is encoded by the?HSD11B2?gene. There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

    Molekulargewicht

    43 kDa

    Gen-ID

    3291

    UniProt

    P80365

    Pathways

    Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones
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