KAP1 Antikörper (AA 699-835)

Produktnummer ABIN3043491

Dieser Anti-KAP1-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von KAP1 in WB, IHC, IF, FACS und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für KAP1 Antikörper (AA 699-835) (ABIN3043491)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-KAP1 Antikörper

Bindungsspezifität: AA 699-835

Verwendungszweck: Anti-KAP1/TRIM28 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-KAP1/TRIM28 Antibody (ABIN3043491). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: TRIM28

Hintergrund:

Synonyms: Transcription intermediary factor 1-beta,TIF1-beta,E3 SUMO-protein ligase TRIM28,6.3.2.-,KRAB-associated protein 1,KAP-1,KRAB-interacting protein 1,KRIP-1,Nuclear corepressor KAP-1,RING finger protein 96,Tripartite motif-containing protein 28,TRIM28,KAP1, RNF96, TIF1B,

Tissue Specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. .

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Molekulargewicht: 100 kDa

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus