PDK4 Antikörper (N-Term)

Produktnummer ABIN3043461

Dieses Anti-PDK4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PDK4 in WB. Geeignet für Human und Ratte.

Kurzübersicht für PDK4 Antikörper (N-Term) (ABIN3043461)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PDK4 Antikörper

Bindungsspezifität: AA 91-125, N-Term

Verwendungszweck: Anti-PDK4 Antibody Picoband®

Sequenz: WYIQSLMDLV EFHEKSPDDQ KALSDFVDTL IKVRN

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PDK4 Antibody Picoband® (ABIN3043461). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PDK4, different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: PDK4 pyruvate dehydrogenase kinase, isozyme 4". 2. Andrews MT, Squire TL, Bowen CM, Rollins MB (Jul 1998). "Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal". Proceedings of the National Academy of Sciences of the United States of America 95 (14): 8392-7. 3. Gudi R, Bowker-Kinley MM, Kedishvili NY, Zhao Y, Popov KM (Dec 1995). "Diversity of the pyruvate dehydrogenase kinase gene family in humans".The Journal of Biological Chemistry 270 (48): 28989-94.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Andere Bezeichnung: PDK4

Hintergrund:

Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial,2.7.11.2,Pyruvate dehydrogenase kinase isoform 4,PDK4,PDHK4,

Tissue Specificity: Ubiquitous, highest levels of expression in heart and skeletal muscle. .

Background: Pyruvate dehydrogenase lipoamide kinase isozyme 4, mitochondrial is an enzyme that in humans is encoded by the PDK4 gene. This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. In addition, PDK4 is increased in hibernation and helps to decrease metabolism and conserve glucose by decreasing its conversion to acetyl-CoA, which enters the citric acid cycle and is converted to ATP.

Molekulargewicht: 46 kDa

Gen-ID: 5166

UniProt: Q16654

Pathways: PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt