Src Antikörper (AA 2-368)

Produktnummer ABIN3043441

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Src in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für Src Antikörper (AA 2-368) (ABIN3043441)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Src Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Src Antikörper

Bindungsspezifität: AA 2-368

Verwendungszweck: Anti-Src Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Src Antibody (ABIN3043441). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Src recombinant protein (Position: G2-D368). Human Src shares 97% and 99% amino acid (aa) sequences identity with mouse and rat Src, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Src Antikörper (ABIN3043441)

Zhang, Zhou, Lei, Zheng, Chen, Gou, Gu, He, Lan, Qi, Li, Ding, Qiao, Wang: "Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/?-catenin pathway." in: Oncotarget, Vol. 6, Issue 5, pp. 3123-35, (2015) (PubMed).

Details zu Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Andere Bezeichnung: SRC

Hintergrund:

Synonyms: Proto-oncogene tyrosine-protein kinase Src,2.7.10.2,Proto-oncogene c-Src,pp60c-src,p60-Src,SRC,SRC1,

Tissue Specificity: Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Background: Sarcoma (Scr) is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human SRC protooncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Molekulargewicht: 60 kDa

Gen-ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling