Src Antikörper (AA 2-368)

Produktnummer ABIN3043441

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Src in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für Src Antikörper (AA 2-368) (ABIN3043441)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Src Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Src Antikörper

Bindungsspezifität: AA 2-368

Verwendungszweck: Anti-Src Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Src Antibody Picoband® (ABIN3043441). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Src recombinant protein (Position: G2-D368). Human Src shares 97% and 99% amino acid (aa) sequences identity with mouse and rat Src, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Nobel Prize in Physiology or Medicine for 1989 jointly to J. Michael Bishop and Harold E. Varmus for their discovery of "the cellular origin of retroviral oncogenes". Press Release. 2. Entrez Gene: SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)". 3. Gibbs, C. P., Tanaka, A., Anderson, S. K., Radul, J., Baar, J., Ridgway, A., Kung, H.-J., Fujita, D. J.Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of Rous sarcoma virus.J. Virol.53: 19-24, 1985.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Src Antikörper (ABIN3043441)

Zhang, Zhou, Lei, Zheng, Chen, Gou, Gu, He, Lan, Qi, Li, Ding, Qiao, Wang: "Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/?-catenin pathway." in: Oncotarget, Vol. 6, Issue 5, pp. 3123-35, (2015) (PubMed).

Details zu Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Andere Bezeichnung: SRC

Hintergrund:

Synonyms: Proto-oncogene tyrosine-protein kinase Src,2.7.10.2,Proto-oncogene c-Src,pp60c-src,p60-Src,SRC,SRC1,

Tissue Specificity: Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Background: Sarcoma (Scr) is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human SRC protooncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Molekulargewicht: 60 kDa

Gen-ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling