Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PARP1 Antikörper (AA 670-858)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PARP1 in WB, IHC, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN3043378

Kurzübersicht für PARP1 Antikörper (AA 670-858) (ABIN3043378)

Target

Alle PARP1 Antikörper anzeigen
PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität

  • 243
  • 65
  • 50
  • 10
  • 7
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 227
  • 39
  • 1
Kaninchen

Klonalität

  • 211
  • 56
Polyklonal

Konjugat

  • 164
  • 16
  • 15
  • 13
  • 12
  • 12
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser PARP1 Antikörper ist unkonjugiert

Applikation

  • 175
  • 125
  • 69
  • 55
  • 44
  • 41
  • 40
  • 38
  • 24
  • 17
  • 16
  • 7
  • 7
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 16
    • 15
    • 15
    • 12
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 670-858

    Verwendungszweck

    Anti-PARP/PARP1 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-PARP/PARP1 Antibody Picoband® (ABIN3043378). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry , 2 μg/mL, Human, Rat
    Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Ambrose, H. E., Willimott, S., Beswick, R. W., Dantzer, F., Menissier de Murcia, J., Yelamos, J., Wagner, S. D. Poly(ADP-ribose) polymerase-1 (Parp-1)-deficient mice demonstrate abnormal antibody responses. Immunology 127: 178-186, 2008. 2. Doege, C. A., Inoue, K., Yamashita, T., Rhee, D. B., Travis, S., Fujita, R., Guarnieri, P., Bhagat, G., Vanti, W. B., Shih, A., Levine, R. L., Nik, S., Chen, E. I., Abeliovich, A. Early-stage epigenetic modification during somatic cell reprogramming by Parp1 and Tet2. Nature 488: 652-655, 2012.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Liu, Yang, Mu, Fu: "Epigallocatechin-3-Gallate Ameliorates Glucocorticoid-Induced Osteoporosis of Rats in Vivo and in Vitro." in: Frontiers in pharmacology, Vol. 9, pp. 447, (2018) (PubMed).

    Li, Li, Xu, Guo, Du: "Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia." in: International journal of molecular sciences, Vol. 11, Issue 11, pp. 4580-90, (2010) (PubMed).

  • Target

    PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

    Andere Bezeichnung

    PARP1

    Hintergrund

    Synonyms: Poly [ADP-ribose] polymerase 1,PARP-1,2.4.2.30,ADP-ribosyltransferase diphtheria toxin-like 1,ARTD1,NAD (+) ADP-ribosyltransferase 1,ADPRT 1,Poly[ADP-ribose] synthase 1,PARP1,ADPRT, PPOL,

    Tissue Specificity: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. .

    Background: Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

    Sequence Similarities: Contains 1 BRCT domain.

    Molekulargewicht

    120 kDa

    Gen-ID

    142

    UniProt

    P09874

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Sie sind hier:
Chat with us!