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Dystrophin Antikörper (AA 3076-3404)

Dieses Anti-Dystrophin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Dystrophin in IHC, WB, ICC, IHC (fro) und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN3043317

Kurzübersicht für Dystrophin Antikörper (AA 3076-3404) (ABIN3043317)

Target

Alle Dystrophin (DMD) Antikörper anzeigen
Dystrophin (DMD)

Reaktivität

  • 107
  • 16
  • 14
  • 2
Human, Maus, Ratte

Wirt

  • 73
  • 36
Kaninchen

Klonalität

  • 83
  • 26
Polyklonal

Konjugat

  • 51
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser Dystrophin Antikörper ist unkonjugiert

Applikation

  • 82
  • 50
  • 31
  • 16
  • 15
  • 12
  • 6
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 56
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 3076-3404

    Verwendungszweck

    Anti-Dystrophin/DMD Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Dystrophin/DMD Antibody Picoband® (ABIN3043317). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human Dystrophin recombinant protein (Position: H3076-D3404). Human Dystrophin shares 100% amino acid (aa) sequence identity with mouse Dystrophin.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry, 0.5-1 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Tennyson CN, Klamut HJ, Worton RG (1995). "The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced". Nature Genetics 9 (2): 184-90. 2. García-Pelagio KP, Bloch RJ, Ortega A, González-Serratos H (2011). "Biomechanics of the sarcolemma and costameres in single skeletal muscle fibers from normal and dystrophin- null mice". J Muscle Res Cell Motil 31 (5-6): 323-336. 3. Strachan T and Read AP, 1999. Human molecular genetics, BIOS Scientific, New York, USA

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Dystrophin (DMD)

    Andere Bezeichnung

    DMD

    Hintergrund

    Synonyms: Dystrophin,DMD,

    Tissue Specificity: Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver. .

    Background: Dystrophin, also known as DMD, is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is mapped to Xp21.2-p21.1. This complex is variously known as the costamere or thedystrophin-associated protein complex. Many muscle proteins, such as α-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. Dystrophin is a protein located between the sarcolemma and the outermost layer of myofilaments in the muscle fiber (myofiber). It is a cohesive protein, linking actin filaments to another support protein that resides on the inside surface of each muscle fiber's plasma membrane (sarcolemma).

    Sequence Similarities: Contains 2 CH (calponin-homology) domains.

    Molekulargewicht

    427 kDa

    Gen-ID

    1756

    UniProt

    P11532

    Pathways

    Skeletal Muscle Fiber Development
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