Leptin Antikörper (Middle Region)

Produktnummer ABIN3043294

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Leptin in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Maus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für Leptin Antikörper (Middle Region) (ABIN3043294)

Target: Leptin (LEP)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Leptin Antikörper

Bindungsspezifität: AA 74-109, Middle Region

Verwendungszweck: Anti-Leptin Antibody

Sequenz: KMDQTLAVYQ QVLTSLPSQN VLQIANDLEN LRDLLH

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: (ABIN3043294). Tested in ELISA, WB applications. This antibody reacts with Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin, different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Leptin Antikörper (ABIN3043294)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Yuan, Zhang, Cai, Ding, Wang, Chen, Wang, Yan, Lu: "Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer." in: Oncology reports, Vol. 29, Issue 6, pp. 2291-6, (2013) (PubMed).

Cheng, Dai, Dai: "Testis dysfunction by isoproterenol is mediated by upregulating endothelin receptor A, leptin and protein kinase Cvarepsilon and is attenuated by an endothelin receptor antagonist CPU0213." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 29, Issue 4, pp. 421-6, (2010) (PubMed).

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Lep

Hintergrund:

Synonyms: Leptin,Obesity factor,Lep,Ob,

Tissue Specificity: Detected in blood plasma (PubMed:8820107). Produced and secreted by astrocytes (at protein level) (PubMed:19065001). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. .

Background: Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molekulargewicht: 16 kDa

Gen-ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process