CDKN1B Antikörper (AA 10-198)

Produktnummer ABIN3042744

Dieses Anti-CDKN1B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDKN1B in WB, IF, FACS und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für CDKN1B Antikörper (AA 10-198) (ABIN3042744)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-CDKN1B Antikörper

Bindungsspezifität: AA 10-198

Verwendungszweck: Anti-p27 KIP 1/CDKN1B Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-p27 KIP 1/CDKN1B Antibody Picoband® (ABIN3042744). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Polyak, K., Lee, M.-H., Erdjument-Bromage, H., Koff, A., Roberts, J. M., Tempst, P., Massague, J. Cloning of p27(Kip1), a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78: 59-66, 1994. 2. Hauck, L., Harms, C., An, J., Rohne, J., Gertz, K., Dietz, R., Endres, M., von Harsdorf, R.Protein kinase CK2 links extracellular growth factor signaling with the control of p27(Kip1) stability in the heart. Nature Med. 14: 315-324, 2008. Note: Erratum: Nature Med. 14: 585 only, 2008.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-CDKN1B Antikörper (ABIN3042744)

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Wang, Zhao, Wu, Kong, Zhang, Wu, Li, Zhang, Yu, Jin: "Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells." in: Virology journal, Vol. 8, pp. 231, (2011) (PubMed).

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: CDKN1B

Hintergrund:

Synonyms: Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,p27Kip1,CDKN1B,KIP1,

Tissue Specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Background: Cyclin-dependent kinase inhibitor 1B (p27KIP1), also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kipfamily of cyclin dependent kinase (Cdk) inhibitor proteins. It is mapped to 12p13.1. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of p27KIP1 is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

Sequence Similarities: Belongs to the CDI family.

Molekulargewicht: 27 kDa

Gen-ID: 1027

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie