Caspase 1 Antikörper (AA 249-262)

Produktnummer ABIN3030288

Dieses Anti-Caspase 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 1 in WB und IHC (p). Geeignet für Human.

Kurzübersicht für Caspase 1 Antikörper (AA 249-262) (ABIN3030288)

Target: Caspase 1 (CASP1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Caspase 1 Antikörper

Bindungsspezifität: AA 249-262

Aufreinigung: Antigen affinity

Immunogen: Amino acids 249-262 (VFIGRLIEHMQEYA) from the large subunit were used as the immunogen for this Caspase-1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the Caspase-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Caspase 1

Target: Caspase 1 (CASP1)

Andere Bezeichnung: Caspase-1

Hintergrund: Caspase-1, an apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL-18, and IL-33 precursor proteins. It was purified from the cytosol of the THP monocytic cell line and found that the active protease was made up of two peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The CASP1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal metaphase chromosomes. Caspase-1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome