ATM Antikörper (N-Term)

Produktnummer ABIN3030032

Der Kaninchen Polyklonal Anti-ATM-Antikörper wurde für WB validiert. Er ist geeignet, ATM in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ATM Antikörper (N-Term) (ABIN3030032)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ATM Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human ATM (DPETIKHLDRHSDSK) was used as the immunogen for this ATM antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the ATM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the ATM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM

Hintergrund: Ataxia telangiectasia mutated,also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. Linkage analysis of ataxia-telangiectasia led to mapping of the gene to chromosome 11q22.3. Using an antiserum developed to a peptide corresponding to the deduced amino acid sequence of ATM, the protein is a single, high molecular weight protein predominantly confined to the nucleus of human fibroblasts, although it is present in both nuclear and microsomal fractions from human lymphoblast cells and peripheral blood lymphocytes. Overexpression of ATM cDNA in AT cells enhanced their survival after radiation exposure, decreased radiation-induced chromosome aberrations, reduced radioresistant DNA synthesis, and partially corrected defective cell cycle checkpoints and induction of stress-activated protein kinase. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors, in a telomere-independent manner. It functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus