TICAM2 Antikörper (C-Term)

Produktnummer ABIN3029187

Dieses Anti-TICAM2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TICAM2 in WB, ICC, IHC (p) und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für TICAM2 Antikörper (C-Term) (ABIN3029187)

Target: TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TICAM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-TICAM2 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Translocating chain-associating membrane protein 1 (KFINFQLRRWREHSAFQA) was used as the immunogen for this TRAM antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the TRAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the TRAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu TICAM2

Target: TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))

Andere Bezeichnung: TRAM

Hintergrund: By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95 % amino acid identity with the canine protein. Functional analysis indicated that the protein influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

UniProt: Q15629

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades