PCNA Antikörper

Produktnummer ABIN3026537

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PCNA in WB, ELISA, IF, FACS, IHC (p) und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster und Schizosaccharomyces pombe.

Kurzübersicht für PCNA Antikörper (ABIN3026537)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon: PC10

Produktdetails anti-PCNA Antikörper

Produktmerkmale: This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: This antibody recognizes a non-histone protein of 36  kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Gen-ID: 5111

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie