CDK1 Antikörper

Produktnummer ABIN3026474

Dieser Maus Monoklonal Antikörper detektiert spezifisch CDK1 in IF, IHC (p) und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Affe, Rind (Kuh) und Nerz.

Kurzübersicht für CDK1 Antikörper (ABIN3026474)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Affe, Rind (Kuh), Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: POH-1

Produktdetails anti-CDK1 Antikörper

Keine Kreuzreaktivität: Maus, Ratte (Rattus), Xenopus laevis, Fruchtfliege (Drosophila melanogaster), Schizosaccharomyces pombe, Yeast (Saccharomyces cerevisiae)

Produktmerkmale: This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Aufreinigung: Protein G purified antibody

Immunogen: Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this Cdk1 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdk1 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE) only: 1-2 μg/mL (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cdk1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: Cdk1

Hintergrund: This antibody recognizes a 34  kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Gen-ID: 983

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA