GRP94 Antikörper

Produktnummer ABIN3025969

Dieses Anti-GRP94-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von GRP94 in WB, FACS, IF und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Huhn, Hamster, Hund, Kaninchen, Schwein, Meerschweinchen, Affe, Schaf, Pferd und Xenopus laevis.

Kurzübersicht für GRP94 Antikörper (ABIN3025969)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Hamster, Hund, Kaninchen, Schwein, Meerschweinchen, Affe, Schaf, Pferd, Xenopus laevis

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 9G10-F8-2

Produktdetails anti-GRP94 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: GRP94

Hintergrund: Recognizes a protein of 94  kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades