PCNA Antikörper

Produktnummer ABIN3025663

Dieses Anti-PCNA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PCNA in WB, IF, FACS und IHC (p). Geeignet für Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio) und Drosophila melanogaster.

Kurzübersicht für PCNA Antikörper (ABIN3025663)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM350

Produktdetails anti-PCNA Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Recognizes a non-histone protein of 36  kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie