TRIM29 Antikörper (AA 1-200)

Produktnummer ABIN3025033

Der Maus Monoklonal anti-TRIM29 Antikörper (Klon TRIM29-1041) (ABIN3025033) detektiert spezifisch TRIM29 in ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TRIM29 Antikörper (AA 1-200) (ABIN3025033)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRIM29 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: TRIM29-1041

Produktdetails anti-TRIM29 Antikörper

Bindungsspezifität: AA 1-200

Verwendungszweck: TRIM29 Antibody (azide and preservative free)

Produktmerkmale: It recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment (126 amino acid residues between aa 1-200) of human TRIM29 protein was used as the immunogen for the TRIM29 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TRIM29 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TRIM29 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Andere Bezeichnung: TRIM29

Hintergrund: It recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

UniProt: Q14134

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus