GRP94 Antikörper

Produktnummer ABIN3024904

Dieses Anti-GRP94-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von GRP94 in WB, IF und IHC (p). Geeignet für Human.

Kurzübersicht für GRP94 Antikörper (ABIN3024904)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SRPR90b

Produktdetails anti-GRP94 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Titering of the GRP94 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: GRP94

Hintergrund: Heat shock protein 90  kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades