GFM1 Antikörper

Produktnummer ABIN3023784

Der Maus Monoklonal Anti-GFM1-Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, GFM1 in Proben von Human zu detektieren.

Kurzübersicht für GFM1 Antikörper (ABIN3023784)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AE-1

Produktdetails anti-GFM1 Antikörper

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Aufreinigung: Protein G affinity chromatography

Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order Ab without BSA,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Andere Bezeichnung: Mitochondrial

Hintergrund: This mAb recognizes a 60  kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.