ATM Antikörper (AA 980-1512)

Produktnummer ABIN302280

Der Maus Monoklonal anti-ATM Antikörper (Klon 3-00E-008) (ABIN302280) detektiert spezifisch ATM in WB, IP und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ATM Antikörper (AA 980-1512) (ABIN302280)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 3-00E-008

Produktdetails anti-ATM Antikörper

Bindungsspezifität: AA 980-1512

Spezifität: This antibbody detects Ataxia Telangiectasia Mutated (ATM).

Produktmerkmale: Synonyms: Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATDC, TEL1, TELO1

Aufreinigung: Protein G chromatography

Immunogen: Recombinant protein expressed in E. coli corresponding to amino acids 980-1512

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immonoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM

Hintergrund: ATM-3E8 recognizes full-length ATM, a 370 kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. The ATM gene product is a 370 kD nuclear phosphoprotein with C-terminal homology to phosphatidylinositol 3-kinase. ATM is involved in the induction of a DNA damage control pathway. Mutations in the ATM gene cause neuronal degeneration, gonadal abnormalities, and immune deficiencies as part of the hereditary disease ataxia telangiectasia.Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

Gen-ID: 472

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus