Cyclin D1 Antikörper

Produktnummer ABIN302101

Produktdetails anti-Cyclin D1 Antikörper

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Kreuzreaktivität (Details): Human, Rat, Other not determined

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified cyclin D1 protein

Klon: CD1-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/mL, positive tissue: colon.
Western blotting: Recommended dilution: 1 μg/mL.
Immunocytochemistry: Recommended dilution: 1 μg/mL.
Immunoprecipitation: Recommended dilution: 1 μg/mL.
Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.
Flow cytometry: Intracellular staining, membrane permeabilization is required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1 (CCND1 Produkte)

Hintergrund: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

Gen-ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling