RFP Antikörper

Produktnummer ABIN293074

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RFP in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Discosoma.

Kurzübersicht für RFP Antikörper (ABIN293074)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-RFP Antikörper

Verwendungszweck: Anti-RFP / Red Fluorescent Protein Antibody

Aufreinigung: Immunoaffinity purified

Immunogen: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:700000), IHC, WB (1:2000 - 1:10000)

Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C.

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP