RIPK3 Antikörper (AA 480-530)

Produktnummer ABIN291732

Der Kaninchen Polyklonal anti-RIPK3 Antikörper (ABIN291732) detektiert spezifisch RIPK3 in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für RIPK3 Antikörper (AA 480-530) (ABIN291732)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RIPK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-RIPK3 Antikörper

Bindungsspezifität: AA 480-530

Spezifität: A portion of amino acids 480-530 of human RIP3.

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: A synthetic peptide (QEGPKDPEAWSRPQ) corresponding to amino acids 497-510 (isoform CRA_a, 518 amino acids) and 298-311 (isoform CRA_b, 319 amino acids) of human RIP3 was used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Andere Bezeichnung: RIPK3 / RIP3

Hintergrund: Name/Gene ID: RIPK3
Subfamily: RIP
Family: Protein Kinase

Synonyms: RIPK3, Receptor interacting protein 3, RIP-like protein kinase 3, RIP3, RIP-3, Receptor-interacting protein 3

Gen-ID: 11035

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades