TGFB1 Antikörper (Biotin)

Produktnummer ABIN290963

Der Huhn Polyklonal Anti-TGFB1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, TGFB1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für TGFB1 Antikörper (Biotin) (ABIN290963)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Human, Maus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser TGFB1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TGFB1 Antikörper (Biotin)

Spezifität: Recognizes human TGFb1. When used in combination with the monoclonal capture antibody LS-C10854 in a sandwich ELISA, ~5 % cross-reactivity with TGF-b1.2 and 1 % cross-reactivity with rhTGF-b2, rcTGF-b3 and raTG-b5 was observed.

Aufreinigung: Affinity purified

Immunogen: Recombinant human TGF-b1 expressed in CHO cells.

Type of Immunogen: Recombinant protein

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Plates are coated with 100 μL/well of the capture antibody in combination with 100 μL/well of the detection antibody at 300 ng/mL. An ELISA for sample volumes of 100 μL can be obtained. Titrate each preparation of the recombinant protein for standard preparation to arrive at the most suitable dose range. For this antibody pair, a two-fold dilution series starting at 2 ng/mL is suggested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Sterile PBS. Resuspend to 250 μL

Konzentration: Lot specific

Buffer: Supplied as a lyopholized powder from PBS,  pH 7.4, 50 μg BSA.

Handhabung: Avoid frequent freeze thaws. Store undiluted.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGFB1 / TGF Beta 1

Hintergrund: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gen-ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints