Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
Aufreinigung
Affinity chromatography purified
Immunogen
C-myc antibody (HRP) was raised in goat using a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH as the immunogen.
ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
Supplied as a liquid in 50 mM Hepes buffer, pH 7.1, with 0.0 NaCl, with 0.2 % BSA, and 0.01 % thimerasol.
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.