CHEK1 Antikörper (pSer345)

Produktnummer ABIN2857048

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CHEK1 in WB, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für CHEK1 Antikörper (pSer345) (ABIN2857048)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: pSer345

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser345 of human Chk1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr , HCT116 P53 cell

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.66 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-CHEK1 Antikörper (ABIN2857048)

Xu, Zhou, Xiong, Zou, Deng, Ganaie, Kleiboeker, Peng, Liu, Wang, Ye, Qiu: "Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway." in: PLoS pathogens, Vol. 13, Issue 3, pp. e1006266, (2017) (PubMed).

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: checkpoint kinase 1

Hintergrund: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm

Molekulargewicht: 54 kDa

Gen-ID: 1111

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur