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ROCK1 Antikörper (N-Term)

Name: ROCK1 Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN2856887
Price: 659.23 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-ROCK1-Antikörper wurde für WB, IP, IF, IHC (p) und ICC validiert. Er ist geeignet, ROCK1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

WB Image ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. (ROCK1 Antikörper (N-Term))

IHC-P Image ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. (ROCK1 Antikörper (N-Term))

IHC

IP Image ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. (ROCK1 Antikörper (N-Term))

WB Image Sample (30 ug of whole cell lysate) A: A431 , B: H1299 C: Molt-4 , 5% SDS PAGE antibody diluted at 1:1000 (ROCK1 Antikörper (N-Term))

ICC/IF Image ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis. (ROCK1 Antikörper (N-Term))

7 Abbildungen

(1 reference)

Produktnummer ABIN2856887

Kurzübersicht für ROCK1 Antikörper (N-Term) (ABIN2856887)

Target

Alle ROCK1 Antikörper anzeigen

ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser ROCK1 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Güteklasse

KO Validated

Bindungsspezifität
16

16

15

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term

Kreuzreaktivität

Human, Maus, Ratte

Produktmerkmale

Rabbit Polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)
ROCK1 antibody [N1N2], N-term

Aufreinigung

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.

Isotyp

IgG
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1001-1200) antibody
ROCK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510) antibody
ROCK1 Reaktivität: Human WB, ELISA, IF Wirt: Maus Monoclonal 2E2 unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (pSer456), (pThr455) antibody
ROCK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 400-660) antibody
ROCK1 Reaktivität: Human WB, IHC, IP Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody
ROCK1 Reaktivität: Human ELISA Wirt: Maus Monoclonal 1H4 unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 405-668) antibody
ROCK1 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1094-1323) antibody
ROCK1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody
ROCK1 Reaktivität: Human, Maus, Ratte WB, IHC, IP, IF, FACS, ICC Wirt: Kaninchen Monoclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (Internal Region) antibody
ROCK1 Reaktivität: Human, Maus, Ratte, Affe WB, ELISA, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1114-1126) antibody
ROCK1 Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Fledermaus, Hund, Pferd WB Wirt: Maus Monoclonal 154C1465 unconjugated

Applikationshinweise

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: rat brain , NIH-3T3 , C2Cl2 , HeLa
Validation: Comparison, KO/KD

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.35 mg/mL

Buffer

1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Tsuchiya, Hara, Okuda, Itoh, Nishioka, Shiomi, Nagao, Mori, Mori, Ikenouchi, Suzuki, Tanaka, Ohwada, Aoki, Kanagawa, Toda, Nagata, Matsuda, Takayama, Tominaga, Umeda: "Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation." in: Nature communications, Vol. 9, Issue 1, pp. 2049, (2019) (PubMed).
Target

ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Andere Bezeichnung

Rho associated coiled-coil containing protein kinase 1

Molekulargewicht

158 kDa

Gen-ID

6093

UniProt

Q13464

Pathways

Microtubule Dynamics, WNT Signalweg, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling