AKR1C1 Antikörper

Produktnummer ABIN2856139

Produktdetails anti-AKR1C1 Antikörper

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKR1C1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to AKR1C1 (aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase))
AKR1C1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: mouse liver , A549 , AKR1C1-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.6 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-AKR1C1 Antikörper (ABIN2856139)

Zhu, Hu, Zeng, Chang, Ge, Wang, Yan, Zhao, Cao, Ying, Gu, Zheng, He, Yang: "The SIRT2-mediated deacetylation of AKR1C1 is required for suppressing its pro-metastasis function in Non-Small Cell Lung Cancer." in: Theranostics, Vol. 10, Issue 5, pp. 2188-2200, (2020) (PubMed).

Zeng, Zhu, You, Dong, Yang, Zhu, He: "Liquiritin, as a Natural Inhibitor of AKR1C1, Could Interfere With the Progesterone Metabolism." in: Frontiers in physiology, Vol. 10, pp. 833, (2019) (PubMed).

Zhu, Chang, Yan, Hu, Zeng, Zhou, Yuan, Ying, Cao, He, Yang: "AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer." in: Theranostics, Vol. 8, Issue 3, pp. 676-692, (2018) (PubMed).

Details zu AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Andere Bezeichnung: aldo-keto reductase family 1 member C1 (DDH Produkte)

Synonyme: 2-ALPHA-HSD antikoerper, 20-ALPHA-HSD antikoerper, C9 antikoerper, DD1 antikoerper, DD1/DD2 antikoerper, DDH antikoerper, DDH1 antikoerper, H-37 antikoerper, HAKRC antikoerper, HBAB antikoerper, MBAB antikoerper, aldo-keto reductase family 1 member C1 antikoerper, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antikoerper, AKR1C1 antikoerper

Hintergrund: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Cellular Localization: Cytoplasm

Molekulargewicht: 37 kDa

Gen-ID: 1645

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process