NRF2 Antikörper (Internal Region)

Produktnummer ABIN2855612

Der Kaninchen Polyklonal Anti-NRF2-Antikörper wurde für WB, IF, IP, ICC, IHC (p) und ChIP validiert. Er ist geeignet, NRF2 in Proben von Human und Ratte zu detektieren. Es sind 9+ Publikationen verfügbar.

Kurzübersicht für NRF2 Antikörper (Internal Region) (ABIN2855612)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-NRF2 Antikörper

Bindungsspezifität: Internal Region

Produktmerkmale: Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)
NRF2 antibody [N2C2], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.51 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NRF2 Antikörper (ABIN2855612)

Tsai, Chen, Lin, Chou, Chen, Ho, Lin, Hwang: "Miconazole Contributes to NRF2 Activation by Noncanonical P62-KEAP1 Pathway in Bladder Cancer Cells." in: Drug design, development and therapy, Vol. 14, pp. 1209-1218, (2020) (PubMed).

Batubara, Astuti, Prastya, Ilmiawati, Maeda, Suzuki, Hamamoto, Takemori: "The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Chou, Lin, Dzhagalov, Chen, Lin, Lin, Chen, Chen, Fu: "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy." in: Scientific reports, Vol. 10, Issue 1, pp. 8422, (2020) (PubMed).

Neitzel, Seiwert, Göder, Diehl, Weber, Nagel, Stroh, Rasenberger, Christmann, Fahrer: "Lipoic Acid Synergizes with Antineoplastic Drugs in Colorectal Cancer by Targeting p53 for Proteasomal Degradation." in: Cells, Vol. 8, Issue 8, (2020) (PubMed).

Luo, Shang, Brooks, Jiagge, Zhu, Buschhaus, Conley, Fath, Davis, Gheordunescu, Wang, Harouaka, Lozier, Triner, McDermott, Merajver, Luker, Spitz, Wicha: "Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling." in: Cell metabolism, Vol. 28, Issue 1, pp. 69-86.e6, (2019) (PubMed).

García Trejo, Arellano Buendía, Sánchez Reyes, García Arroyo, Arguello García, Loredo Mendoza, Tapia, Sánchez Lozada, Osorio Alonso: "The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).

Fragoulis, Siegl, Fendt, Jansen, Soppa, Brandenburg, Pufe, Weis, Wruck: "Oral administration of methysticin improves cognitive deficits in a mouse model of Alzheimer's disease." in: Redox biology, Vol. 12, pp. 843-853, (2018) (PubMed).

García-Trejo, Arellano-Buendía, Argüello-García, Loredo-Mendoza, García-Arroyo, Arellano-Mendoza, Castillo-Hernández, Guevara-Balcázar, Tapia, Sánchez-Lozada, Osorio-Alonso: "Effects of Allicin on Hypertension and Cardiac Function in Chronic Kidney Disease." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 3850402, (2017) (PubMed).

Arellano-Buendía, Tostado-González, García-Arroyo, Cristóbal-García, Loredo-Mendoza, Tapia, Sánchez-Lozada, Osorio-Alonso: "Anti-Inflammatory Therapy Modulates Nrf2-Keap1 in Kidney from Rats with Diabetes." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4693801, (2017) (PubMed).

Details zu NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Andere Bezeichnung: NRF2

Hintergrund: NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Cellular Localization: Cytoplasm , cytosol , Nucleus

Molekulargewicht: 68 kDa110

Gen-ID: 4780

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling