KAP1 Antikörper (N-Term)

Produktnummer ABIN2855332

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KAP1 in WB, IF, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für KAP1 Antikörper (N-Term) (ABIN2855332)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-KAP1 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to KAP1 (tripartite motif-containing 28)
KAP1 antibody [N1N2], N-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human KAP1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Mouse brain , HeLa , HepG2 , NIH-3T3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.31 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-KAP1 Antikörper (ABIN2855332)

Ochiai, Hayashi, Umeda, Yoshimura, Harada, Shimizu, Nakano, Saitoh, Liu, Yamamoto, Okamura, Ohkawa, Kimura, Nikaido: "Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells." in: Science advances, Vol. 6, Issue 25, pp. eaaz6699, (2020) (PubMed).

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: tripartite motif containing 28

Hintergrund: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Cellular Localization: Nucleus (By similarity)

Molekulargewicht: 89 kDa

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus