KAP1 Antikörper

Produktnummer ABIN2855331

Dieses Anti-KAP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von KAP1 in WB, IF, IP, ICC, IHC (p) und ChIP. Geeignet für Human. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für KAP1 Antikörper (ABIN2855331)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-KAP1 Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)
KAP1 antibody [N3C2], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.48 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-KAP1 Antikörper (ABIN2855331)

Liu, Li, Liu, Li, Wei, Liu, Lv: "Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus." in: Pathology oncology research : POR, Vol. 25, Issue 4, pp. 1645-1652, (2020) (PubMed).

Hung, Johnson, Chen, Byrum, Bredemeyer, Yewdell, Johnson, Lee, Deivasigamani, Hindi, Amatya, Gross, Paull, Pisapia, Chaudhuri, Petrini, Mosammaparast, Amarasinghe, Zha, Tyler, Sleckman: "MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining." in: Molecular cell, Vol. 71, Issue 2, pp. 332-342.e8, (2019) (PubMed).

Wang, Wolgemuth: "BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis." in: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1429-38, (2016) (PubMed).

Aslanian, Yates, Hunter: "Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells." in: DNA repair, Vol. 15, pp. 29-38, (2014) (PubMed).

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: tripartite motif containing 28

Hintergrund: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Cellular Localization: Nucleus (By similarity)

Molekulargewicht: 89 kDa

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus