SQSTM1 Antikörper

Produktnummer ABIN2854836

Dieses Anti-SQSTM1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SQSTM1 in WB, IF, IP, ICC, FACS, IHC (p) und PLA. Geeignet für Human. Dieses Primary Antibody wurde in 13+ Publikationen zitiert.

Kurzübersicht für SQSTM1 Antikörper (ABIN2854836)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SQSTM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Güteklasse: KO Validated

Produktdetails anti-SQSTM1 Antikörper

Kreuzreaktivität: Bee, Rind (Kuh), Human, Moskito, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
SQSTM1 antibody [N3C1], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2

Validation: Comparison, KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.39 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-SQSTM1 Antikörper (ABIN2854836)

Liu, Jiang, Wu, Wu, Wu, Zhao, Sun, Huang, Li, Sheng, Barbier, Cintrat, Gillet, Su: "Antiviral Effects of ABMA and DABMA against Influenza Virus In Vitro and In Vivo via Regulating the Endolysosomal Pathway and Autophagy." in: , Vol. 23, Issue 7, (2022) (PubMed).

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Chou, Lin, Dzhagalov, Chen, Lin, Lin, Chen, Chen, Fu: "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy." in: Scientific reports, Vol. 10, Issue 1, pp. 8422, (2020) (PubMed).

Jensen, Schuldi, McAvoy, Russell, Boehringer, Curran, Krishnamurthy, Wen, Westergard, Ma, Haeusler, Edbauer, Pasinelli, Trotti: "Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment." in: EMBO molecular medicine, Vol. 12, Issue 5, pp. e10722, (2020) (PubMed).

Martello, Lauriola, Mellis, Parish, Dawson, Imrie, Vidmar, Gammoh, Mitić, Brittan, Mills, Carragher, DArca, Caporali: "Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy." in: EMBO reports, Vol. 21, Issue 7, pp. e48192, (2020) (PubMed).

Wong, Chiu, Tu, Sheu, Chan: "Anticancer Effects of Antihypertensive L-Type Calcium Channel Blockers on Chemoresistant Lung Cancer Cells via Autophagy and Apoptosis." in: Cancer management and research, Vol. 12, pp. 1913-1927, (2020) (PubMed).

Hsieh, Li, Hsu, Chen, Chen, Liu, Liu, Kuo, Liu: "Mitochondrial protection by simvastatin against angiotensin II-mediated heart failure." in: British journal of pharmacology, Vol. 176, Issue 19, pp. 3791-3804, (2020) (PubMed).

Nombela, Requena-Platek, Morales-Lange, Chico, Puente-Marin, Ciordia, Mena, Coll, Perez, Mercado, Ortega-Villaizan: "Rainbow Trout Red Blood Cells Exposed to Viral Hemorrhagic Septicemia Virus Up-Regulate Antigen-Processing Mechanisms and MHC I&II, CD86, and CD83 Antigen-presenting Cell Markers." in: Cells, Vol. 8, Issue 5, (2019) (PubMed).

Lin, Lin, Chen, Yang, Hung: "Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 1, (2019) (PubMed).

Chiu, Hsu, Hsu, Huang, Liu, Wu, Huang, Chen, Chen, Chiu: "Human non‑small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy." in: International journal of oncology, Vol. 53, Issue 5, pp. 1967-1979, (2019) (PubMed).

Yi, Wang, Du, He, Ho, Qiu, Zou, Li, Jin, Zhan, Zhao, Liu: "Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells." in: Molecules (Basel, Switzerland), Vol. 23, Issue 6, (2018) (PubMed).

Zheng, Li, Li, Zhu, Cui, Wong, Chang, Liao: "Induction of N-Ras degradation by flunarizine-mediated autophagy." in: Scientific reports, Vol. 8, Issue 1, pp. 16932, (2018) (PubMed).

Chiu, Hu, Yang, Hsin, Ko, Tsai, Sheu: "Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125774, (2015) (PubMed).

Details zu SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Andere Bezeichnung: sequestosome 1

Hintergrund: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Cellular Localization: Cytoplasm , Late endosome , Nucleus

Molekulargewicht: 48 kDa

Gen-ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Autophagie