CDKN1B Antikörper

Produktnummer ABIN2854753

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CDKN1B in WB, IHC, IF, IHC (p), IP, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für CDKN1B Antikörper (ABIN2854753)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse: KO Validated

Produktdetails anti-CDKN1B Antikörper

Spezifität: IP/MS validation was supported by references (PMID:30377371)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
p27 Kip1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Validation: IP/MS, KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.08 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-CDKN1B Antikörper (ABIN2854753)

Scully, Lahmy, Signaevskaia, Sasik, Medal, Kim, French, James, Wu, Lowy, Itkin-Ansari: "E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53." in: Cellular and molecular gastroenterology and hepatology, Vol. 6, Issue 2, pp. 181-198, (2018) (PubMed).

Mlcochova, Caswell, Taylor, Towers, Gupta: "DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages." in: The EMBO journal, Vol. 37, Issue 1, pp. 50-62, (2018) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: cyclin dependent kinase inhibitor 1B

Hintergrund: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Cellular Localization: Nucleus , Cytoplasm

Molekulargewicht: 22 kDa

Gen-ID: 1027

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie