Cyclin H Antikörper (Middle Region)

Produktnummer ABIN2792693

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cyclin H in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Meerschweinchen, Kaninchen, Pferd und Rind (Kuh).

Kurzübersicht für Cyclin H Antikörper (Middle Region) (ABIN2792693)

Target: Cyclin H (CCNH)

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Kaninchen, Pferd, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Cyclin H Antikörper

Bindungsspezifität: Middle Region

Sequenz: KQKLERCHSA ELALNVITKK RKGYEDDDYV SKKSKHEEEE WTDDDLVESL

Kreuzreaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte (Rattus)

Homologie: Cow: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 78%, Rat: 85%

Produktmerkmale: This is a rabbit polyclonal antibody against CCNH. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CCNH

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-CCNH Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: MCF7 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 323 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu Cyclin H

Target: Cyclin H (CCNH)

Andere Bezeichnung: Cyclin H

Hintergrund: CCNH belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAK, p34, p37
Protein Interaction Partner: SFN, GOLGA2, SSX2IP, CCDC170, CCDC33, DUSP12, SSSCA1, NDC80, CALCOCO2, PPFIA1, PSMA1, KLC3, RPA3, RPA2, RPA1, NEDD4, CDK7, CDK8, CDK2, CCNC, MNAT1, MBP, CTD, CDK20, CTBP2, CSNK2B, TRIM8, GANAB, RARB, POLR2A, GTF2H4, ERCC2, tat, POLR2B, CDK9, BRCA1, GTF2H1
Protein Size: 323

Molekulargewicht: 38 kDa

Gen-ID: 902

NCBI Accession: NM_001239, NP_001230

UniProt: P51946

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase