EPAS1 Antikörper (Middle Region)

Produktnummer ABIN2779523

Der Kaninchen Polyklonal Anti-EPAS1-Antikörper wurde für WB validiert. Er ist geeignet, EPAS1 in Proben von Human, Ratte, Maus, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Hund und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für EPAS1 Antikörper (Middle Region) (ABIN2779523)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reaktivität: Human, Ratte, Maus, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Hund, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-EPAS1 Antikörper

Bindungsspezifität: Middle Region

Sequenz: ESLFKPHLMA MNSIFDSSGK GAVSEKSNFL FTKLKEEPEE LAQLAPTPGD

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against EPAS1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EPAS1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 870 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Andere Bezeichnung: EPAS1

Hintergrund: EPAS1 is a transcription factor involved in the induction of oxygen regulated genes. EPAS1 binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. EPAS1 regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. EPAS1 may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. EPAS1 is a potent activator of the Tie-2 tyrosine kinase expression. The activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction of EPAS1 with redox regulatory protein APEX seems to activate CTAD.
Alias Symbols: HIF2A, HLF, MOP2, PASD2, ECYT4, bHLHe73
Protein Interaction Partner: MAP1LC3A, SQSTM1, LAMP1, BATF2, BATF3, BATF, DDIT3, DBP, NDN, JUN, EPAS1, NPM1, BBS4, BBS2, BBS1, Arnt, VHL, STAT5A, SMAD3, EWSR1, MED11, MED8, EGLN3, PSMD1, PSMB1, MED1, PHB, MITF, HIF1A, EP300, EMD, CTNNB1, CREBBP, EGLN2, DPY30, MED10, MED25, ARID1B, WR
Protein Size: 870

Molekulargewicht: 90 kDa

Gen-ID: 2034

NCBI Accession: NM_001430, NP_001421

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt