Leptin Antikörper (N-Term)

Produktnummer ABIN2776943

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Leptin in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Pferd, Hund, Ziege, Kaninchen, Schaf und Meerschweinchen.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Leptin Antikörper (N-Term) (ABIN2776943)

Target: Leptin (LEP)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Hund, Ziege, Kaninchen, Schaf, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Leptin Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: LEP Antibody - N-terminal region

Sequenz: MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS

Homologie: Cow: 86%, Dog: 86%, Goat: 91%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%, Sheep: 91%

Produktmerkmale: This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LEP

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41697-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-Leptin Antikörper (ABIN2776943)

Müller, Kowalewski, Reichler, Kollár, Balogh: "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." in: Reproduction in domestic animals = Zuchthygiene, Vol. 52 Suppl 2, pp. 187-192, (2017) (PubMed).

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: LEP

Hintergrund:

Background Information: This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development.

Gene Name: leptin

Alternative Symbols: OB, OBS, LEPD

Protein Name: leptin

Molekulargewicht: 19kDa

Gen-ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process