ROR1 Antikörper

Produktnummer ABIN2689893

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-ROR1 Antikörper wird verwendet zum Nachweis von ROR1 in Proben von Human. Er wurde validiert für FACS, IP, IHC (p) und IHC (fro).

Kurzübersicht für ROR1 Antikörper (ABIN2689893)

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ROR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 4A5

Produktdetails anti-ROR1 Antikörper

Produktmerkmale: The 4A5 monoclonal antibody binds to the glycosylated type I membrane protein ROR1 (Receptor-tyrosine-kinase-like Orphan Receptor 1). The ROR1 and ROR2 related proteins are highly conserved evolutionarily and are primarily expressed during embryogenesis. ROR1 is not expressed on normal peripheral blood lymphocytes. ROR1 is similar to tropomyosin receptor kinase (Trk family) neurotropic receptors and shares a cysteine-rich domain with Frizzled receptors for Wnt-family signaling proteins, which are involved in the regulation of embryogenesis and carcinogenesis. Furthermore, there is mounting evidence that aberrant ROR1 expression contributes to human malignancy. ROR1 expression in human embryonal carcinoma cells. Human NCCIT cells (ATCC CRL-2073) were stained with either Purified Mouse IgG2b κ Isotype Control (Cat. No. 555740, dashed line histogram) or Purified Mouse Anti-Human ROR1 (Cat. No. 564464, solid-line histogram) monoclonal antibodies. The second-step reagent was PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable NCCIT cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

BD Pharmingen™ Purified Mouse Anti-Human ROR1 - Purified - Clone 4A5 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ROR1 cDNA

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-ROR1 Antikörper (ABIN2689893)

Cui, Zhang, Chen, Yu, Widhopf, Fecteau, Rassenti, Kipps: "Targeting ROR1 inhibits epithelial-mesenchymal transition and metastasis." in: Cancer research, Vol. 73, Issue 12, pp. 3649-60, (2013) (PubMed).

Zhang, Chen, Wang-Rodriguez, Zhang, Cui, Frankel, Wu, Kipps: "The onco-embryonic antigen ROR1 is expressed by a variety of human cancers." in: The American journal of pathology, Vol. 181, Issue 6, pp. 1903-10, (2012) (PubMed).

Zhang, Chen, Cui, Chuang, Yu, Wang-Rodriguez, Tang, Chen, Basak, Kipps: "ROR1 is expressed in human breast cancer and associated with enhanced tumor-cell growth." in: PLoS ONE, Vol. 7, Issue 3, pp. e31127, (2012) (PubMed).

Broome, Rassenti, Wang, Meyer, Kipps: "ROR1 is expressed on hematogones (non-neoplastic human B-lymphocyte precursors) and a minority of precursor-B acute lymphoblastic leukemia." in: Leukemia research, Vol. 35, Issue 10, pp. 1390-4, (2011) (PubMed).

Fukuda, Chen, Endo, Tang, Lu, Castro, Widhopf, Rassenti, Cantwell, Prussak, Carson, Kipps: "Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 8, pp. 3047-52, (2008) (PubMed).

Details zu ROR1

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Andere Bezeichnung: ROR1

Hintergrund: Synonyms: Neurotrophic tyrosine kinase, receptor-related 1, NTRKR1

Pathways: RTK Signalweg, WNT Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha