PCNA Antikörper

Produktnummer ABIN2689877

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PCNA in IHC (f), ICS und BI. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für PCNA Antikörper (ABIN2689877)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), BioImaging (BI)

Klon: PC10

Produktdetails anti-PCNA Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation. Human PCNA has been reported to be 262 amino acids with an apparent molecular weight of 36 kDa. Profile of methanol-fixed MOLT-4 cells analyzed on a BD FACScan™ Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-PCNA antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells. The Triton™ X-100 perm protocol is not recommended for use with this antibody. (See Recommended Assay Procedure).

BD Pharmingen™ Purified Mouse Anti-Human PCNA - Purified - Clone PC10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-PCNA Antikörper (ABIN2689877)

Mathews, Bernstein, Franza, Garrels: "Identity of the proliferating cell nuclear antigen and cyclin." in: Nature, Vol. 309, Issue 5966, pp. 374-6, (1984) (PubMed).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie