Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) Antikörper (Biotin)

Details zu Produkt Nr. ABIN2688921
Antigen
  • CD11C
  • SLEB6
  • RGD1561123
  • AI449405
  • Cd11c
  • Cr4
  • N418
  • integrin subunit alpha X
  • integrin alpha X
  • ITGAX
  • Itgax
Reaktivität
Maus
369
124
30
19
18
10
2
2
2
1
Wirt
Armenischer Hamster
276
106
53
37
7
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Biotin
45
45
42
25
14
10
10
7
7
6
6
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Applikation
Flow Cytometry (FACS)
328
123
93
82
67
56
50
37
10
6
6
4
4
4
3
2
1
1
1
1
1
1
Optionen
Marke BD Pharmingen™
Immunogen C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
Klon HL3
Isotyp IgG1, lambda
Produktmerkmale The HL3 antibody reacts with the integrin αx chain of gp150, 95 (CD11c/CD18) which is expressed on dendritic cells and CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and is upregulated on IEL and lymph-node T cells following in vivo activation. CD11c is also found on human NK cells. Although its expression on mouse NK cells is not published, we have detected CD11c on mouse splenic NK cells. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11c on spleen NK cells. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. No. 557391/553165) and isotype control (left panel) or biotin-conjugated mAb HL3 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD11c - Biotin - Clone HL3 - Isotype Armenian Hamster IgG1, λ2 - Reactivity Ms - 0.5 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Antigen
Andere Bezeichnung CD11c (ITGAX Antibody Abstract)
Hintergrund Synonyms: Integrin αx chain
Pathways Activated T Cell Proliferation, Integrin Complex
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Produkt verwendet in: Gao, Liu, Wen, Zhang, Durbin, Liu, Zheng: "Differentiation of monocytic cell clones into CD8 alpha+ dendritic cells (DC) suggests that monocytes can be direct precursors for both CD8 alpha+ and CD8 alpha- DC in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5927-35, 2003 (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, 1997 (PubMed).

Maraskovsky, Brasel, Teepe, Roux, Lyman, Shortman, McKenna: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, 1996 (PubMed).

Huleatt, Lefrançois: "Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5684-93, 1995 (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990 (PubMed).

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