MECP2 Antikörper

Produktnummer ABIN2668837

Es gibt 1 Publikation für dieses Produkt. Der Ratte Monoklonal anti-MECP2 Antikörper wird verwendet zum Nachweis von MECP2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ICC, IP, IF und ChIP.

Kurzübersicht für MECP2 Antikörper (ABIN2668837)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reaktivität: Human, Maus, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser MECP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Klon: 4H7

Produktdetails anti-MECP2 Antikörper

Verwendungszweck: MeCP2 antibody (mAb) (Clone 4H7)

Aufreinigung: Protein G Chromatography

Immunogen: This MeCP2 antibody was raised against a full-length protein corresponding to rat MeCP2.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications: ChIP IP ICC/IF IHC WB View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-MECP2 Antikörper (ABIN2668837)

Jost, Rottach, Milden, Bertulat, Becker, Wolf, Sandoval, Petazzi, Huertas, Esteller, Kremmer, Leonhardt, Cardoso: "Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies." in: PLoS ONE, Vol. 6, Issue 11, pp. e26499, (2011) (PubMed).

Details zu MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Andere Bezeichnung: MeCP2

Hintergrund: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl CpG binding protein 2, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.

Molekulargewicht: 72 kDa

Gen-ID: 29386

NCBI Accession: NP_073164

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane