Rpb1 CTD Antikörper (pSer7)

Produktnummer ABIN2668542

Dieser Ratte Monoklonal Antikörper detektiert spezifisch Rpb1 CTD in WB, IF, ICC, ChIP, ChIP-seq und IP. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 6+ Publikationen zitiert.

Kurzübersicht für Rpb1 CTD Antikörper (pSer7) (ABIN2668542)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

Klon: 4E12

Produktdetails anti-Rpb1 CTD Antikörper

Bindungsspezifität: pSer7

Verwendungszweck: RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)

Aufreinigung: Protein G Chromatography

Immunogen: This RNA pol II CTD phospho Ser7 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ChIP: 5-10 μg per ChIP ChIP-Seq: 5-10 μg each

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Rpb1 CTD Antikörper (ABIN2668542)

Chung, Xu, Fuchs, Mund, Lange, Staege, Schubert, Bian, Dunkel, Eberharter, Regnard, Klinker, Meierhofer, Cozzuto, Winterpacht, Di Croce, Min, Will, Kinkley: "PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3." in: eLife, Vol. 5, (2016) (PubMed).

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Takahashi, Takigawa, Watanabe, Anwar, Shibata, Tomomori-Sato, Sato, Ranjan, Seidel, Tsukiyama, Mizushima, Hayashi, Ohkawa, Conaway, Conaway, Hatakeyama: "MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex." in: Nature communications, Vol. 6, pp. 5941, (2015) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA Pol II CTD

Hintergrund: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 7 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Molekulargewicht: 240 kDa

Gen-ID: 5430

NCBI Accession: NP_000928