Rpb1 CTD Antikörper (pSer2)

Produktnummer ABIN2668545

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Rpb1 CTD in WB und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rpb1 CTD Antikörper (pSer2) (ABIN2668545)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-Rpb1 CTD Antikörper

Bindungsspezifität: pSer2

Verwendungszweck: RNA pol II CTD phospho Ser2 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at serine 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IP: 5 - 10 µL per IP WB: 1:500 - 1:2,000 dilution For RNA pol II CTD phospho Ser2, we also offer AbFlex RNA pol II CTD phospho Ser2 Recombinant Antibody (rAb). For details, see Catalog No. 91115.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA Pol II CTD

Hintergrund: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Molekulargewicht: 240 kDa

Gen-ID: 5430

NCBI Accession: NP_000928