Rpb1 CTD Antikörper (pSer5)

Produktnummer ABIN6972642

Produktdetails anti-Rpb1 CTD Antikörper

Target: Rpb1 CTD (RNA Polymerase II CTD)

Bindungsspezifität: pSer5

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktmerkmale: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA pol II CTD (Rpb1 CTD Produkte)

Synonyme: 5 antikoerper, 8WG16 antikoerper, CG1554 antikoerper, CTD antikoerper, Dmel\\CG1554 antikoerper, H5 antikoerper, II antikoerper, L5 antikoerper, POL antikoerper, Pol II antikoerper, Pol II CTD antikoerper, Pol II Ser5P antikoerper, Pol II0[ser2] antikoerper, Pol II0[ser5] antikoerper, Pol II[ser2] antikoerper, Pol IIa antikoerper, Pol IIo antikoerper, Pol IIo[Ser2] antikoerper, Pol IIo[ser2] antikoerper, Pol IIo[ser5] antikoerper, Pol-IIa antikoerper, PolII antikoerper, PolIIo antikoerper, RNA Pol II antikoerper, RNA Pol II CTD antikoerper, RNA PolI 215 antikoerper, RNA PolII antikoerper, RNA pol II antikoerper, RNA pol IIo antikoerper, RNA polII antikoerper, RNAP antikoerper, RNAP II antikoerper, RNAP II LS antikoerper, RNAPII antikoerper, RNAPII0 antikoerper, RNApol2 antikoerper, RNApolII antikoerper, RPB1 antikoerper, RPII215 antikoerper, RpII antikoerper, Rpb1 antikoerper, Rpll215 antikoerper, Ubl antikoerper, dRPB1 antikoerper, dRpb1 antikoerper, l(1)10Ca antikoerper, l(1)DC912 antikoerper, l(1)DF912 antikoerper, l(1)G0040 antikoerper, l(1)L5 antikoerper, pol II antikoerper, polII antikoerper, RNA polymerase II 215kD subunit antikoerper, RpII215 antikoerper

Molekulargewicht: 210 kDa

NCBI Accession: NP_000928