Rpb1 CTD Antikörper (pSer5)

Produktnummer ABIN2668520

Zitiert in 3+ Publikationen. Der Kaninchen Polyklonal anti-Rpb1 CTD Antikörper (ABIN2668520) detektiert spezifisch Rpb1 CTD in WB, ChIP und ChIP-seq. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rpb1 CTD Antikörper (pSer5) (ABIN2668520)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Rpb1 CTD Antikörper

Bindungsspezifität: pSer5

Verwendungszweck: RNA pol II CTD phospho Ser5 antibody (pAb)

Aufreinigung: Protein A Chromatography

Immunogen: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 4 - 10 μg per ChIP ChIP-Seq: 4 µL each WB: 0.5 - 2 μg/mL dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Rpb1 CTD Antikörper (ABIN2668520)

Young, Hillyer, Hokamp, Fitzpatrick, Konstantinov, Welty, Ness, Werner-Washburne, Fleming, Osley: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." in: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).

Ke, McKnight, Gracey Maniar, Sun, Callaway, Majnik, Lane, Cohen: "IUGR increases chromatin-remodeling factor Brg1 expression and binding to GR exon 1.7 promoter in newborn male rat hippocampus." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 309, Issue 2, pp. R119-27, (2015) (PubMed).

Lee, Chen, Huang, Stargell: "The head module of Mediator directs activation of preloaded RNAPII in vivo." in: Nucleic acids research, Vol. 41, Issue 22, pp. 10124-34, (2013) (PubMed).

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA Pol II CTD

Hintergrund: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Molekulargewicht: 210 kDa

Gen-ID: 5430

NCBI Accession: NP_000928