SIRT1 Antikörper (Sirtuin 1) (AA 581-630)

Details for Product anti-SIRT1 Antibody No. ABIN2668278
Antigen
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • sirtuin 1 L homeolog
  • SIRT1
  • SRT1
  • Sirt1
  • sirt1.L
Epitop
AA 581-630
41
19
18
15
15
10
10
9
8
6
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human
297
142
64
10
7
3
3
3
3
3
3
1
1
Wirt
Maus
236
48
30
5
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser SIRT1 Antikörper ist unkonjugiert
22
12
9
9
9
9
9
9
7
3
3
3
3
3
3
3
2
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
220
122
78
40
40
33
31
13
13
11
6
5
2
2
2
1
1
1
Optionen
Immunogen This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.
Klon 2G1-F7
Isotyp IgG1
Reinigung Protein G Chromatography
Plasmids, Primers & others Plasmide, Primers & weitere SIRT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung SIRT1 (SIRT1 Antibody Abstract)
Hintergrund SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
Molekulargewicht 120 kDa
Gen-ID 23411
Pathways MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 μg/μL
Buffer 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung -20 °C
Informationen zur Lagerung Antibodies in solution can be stored at -20 °C for 2 years.
Haltbarkeit 6 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Sirtuin 1 (SIRT1) (AA 581-630) antibody (ABIN2668278) SIRT1 mAb (Clone 2G1/F7) tested by Western blot. Nuclear extract (20 µg) of CMSL0 cel...
Produkt verwendet in: Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." in: Epigenetics, Vol. 8, Issue 6, pp. 612-23, 2013 (PubMed).

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." in: Molecular cell, Vol. 16, Issue 1, pp. 93-105, 2004 (PubMed).

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