SIRT1 Antikörper (AA 581-630)

Produktnummer ABIN2668278

Dieser Maus Monoklonal Antikörper detektiert spezifisch SIRT1 in WB, IP, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für SIRT1 Antikörper (AA 581-630) (ABIN2668278)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 2G1-F7

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: AA 581-630

Verwendungszweck: SIRT1 antibody (mAb) (Clone 2G1/F7)

Aufreinigung: Protein G Chromatography

Immunogen: This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2.0 μg/mL dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-SIRT1 Antikörper (ABIN2668278)

Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." in: Epigenetics, Vol. 8, Issue 6, pp. 612-23, (2013) (PubMed).

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." in: Molecular cell, Vol. 16, Issue 1, pp. 93-105, (2004) (PubMed).

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1

Hintergrund: SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

Molekulargewicht: 120 kDa

Gen-ID: 23411

NCBI Accession: NP_036370

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling