SIRT1 Antikörper (Sirtuin 1)

Details for Product anti-SIRT1 Antibody No. ABIN969396, Anbieter: Anmelden zum Anzeigen
Antigen
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • sirtuin 1 L homeolog
  • SIRT1
  • SRT1
  • Sirt1
  • sirt1.L
Reaktivität
Human
302
155
70
21
11
10
6
6
6
5
4
4
4
2
2
2
2
Wirt
Maus
207
84
30
5
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser SIRT1 Antikörper ist unkonjugiert
17
14
8
6
6
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
256
144
104
68
64
45
42
40
40
14
6
4
4
2
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Purified recombinant fragment of human SIRT1 expressed in E. Coli.
Klon 1F3
Isotyp IgG1
Reinigung Ascites
Plasmids, Primers & others Plasmide, Primers & weitere SIRT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung SIRT1 (SIRT1 Antibody Abstract)
Hintergrund The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molekulargewicht 120 kDa
Gen-ID 23411
HGNC 23411
Pathways MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
Applikations-hinweise ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung 4°C, -20°C for long term storage
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Sirtuin 1 (SIRT1) antibody (ABIN969396) Figure 1: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-Sirtuin 1 (SIRT1) antibody (ABIN969396) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-Sirtuin 1 (SIRT1) antibody (ABIN969396) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-Sirtuin 1 (SIRT1) antibody (ABIN969396) Figure 4: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), ...
Produkt verwendet in: Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009 (PubMed).

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, 2009 (PubMed).

Haben Sie etwas anderes gesucht?