TGFB1 Antikörper (Biotin)

Produktnummer ABIN2661297

Dieser Maus Monoklonal Antikörper erkennt spezifisch TGFB1 in FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für TGFB1 Antikörper (Biotin) (ABIN2661297)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFB1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: 19D8

Produktdetails anti-TGFB1 Antikörper (Biotin)

Aufreinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C.

Details zu TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGF-Beta1

Hintergrund: TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes. TGF-β1 is synthesized as a 390 amino acid precursor that is cleaved by furin, localized in the trans-Golgi network, in residue 278. Furin processes the TGF-β1 precursor at the carboxyl side of the consensus sequence RHRR which precedes the NH2-terminal Ala 279 residue of the mature TGF-β1. The TGF-β1 precursor includes the latency-associated peptide (LAP dimer) in the N-terminal portion and the 25 kD portion that constitutes the mature TGF-β1 in the C-terminal. LAP dimer and the TGF-β1 mature protein remain non-covalently associated after furin cleavage and this complex does not bind to the TFG-β1 receptor. In addition, the TGF-β1 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active form requires dissociation from LAP. Some activators can release TGF-β1 from LAP such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8. Mouse TGF-β1 converts naive T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF- β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naive CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORgt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORgt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1b, and IL-2 in combination with IL-6, IL-21, or IL-23.

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints