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MAP3K8 Antikörper

MAP3K8 Reaktivität: Human, Ratte, Maus WB, IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN265369
  • Target Alle MAP3K8 Antikörper anzeigen
    MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
    Reaktivität
    • 89
    • 41
    • 39
    • 9
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Ratte, Maus
    Wirt
    • 88
    • 1
    Kaninchen
    Klonalität
    • 88
    • 1
    Polyklonal
    Konjugat
    • 40
    • 8
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MAP3K8 Antikörper ist unkonjugiert
    Applikation
    • 55
    • 41
    • 30
    • 18
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Spezifität
    This antibody detects endogenous levels of MAP3K8 / TPL-2 protein(region surrounding Pro284).
    Kreuzreaktivität (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Aufreinigung
    Affinity Chromatography using epitope-specific immunogen
    Top Product
    Discover our top product MAP3K8 Primärantikörper
  • Applikationshinweise
    ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
    Andere Bezeichnung
    MAP3K8 / TPL-2 (MAP3K8 Produkte)
    Synonyme
    MAP3K8 antikoerper, COT antikoerper, EST antikoerper, ESTF antikoerper, MEKK8 antikoerper, TPL2 antikoerper, Tpl-2 antikoerper, c-COT antikoerper, Cot antikoerper, Cot/Tpl2 antikoerper, Est antikoerper, Estf antikoerper, Tpl2 antikoerper, D17TPL2 antikoerper, mitogen-activated protein kinase kinase kinase 8 antikoerper, MAP3K8 antikoerper, map3k8 antikoerper, Map3k8 antikoerper
    Hintergrund
    The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2
    Molekulargewicht
    approx. 53 kDa
    Gen-ID
    1326
    NCBI Accession
    NP_005195
    UniProt
    P41279
    Pathways
    PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg
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