BAX Antikörper

Produktnummer ABIN265325

Der Kaninchen Polyklonal Anti-BAX-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, BAX in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für BAX Antikörper (ABIN265325)

Target: BAX (BCL2-Associated X Protein (BAX))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-BAX Antikörper

Spezifität: This antibody detects endogenous levels of Bax-α/β/δ/ε/σ protein (region surrounding Ser4).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: Bax

Hintergrund: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4

Molekulargewicht: approx. 21 kDa

Gen-ID: 9606

UniProt: Q07812

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response