XBP1 Antikörper (Internal Region)

Produktnummer ABIN263217

Dieses Anti-XBP1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von XBP1 in WB, ELISA, IHC und IF. Geeignet für Human.

Kurzübersicht für XBP1 Antikörper (Internal Region) (ABIN263217)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser XBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-XBP1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: XBP1 / TREB5

Sequenz: NHSWEDTFAN ELFPQ

Spezifität: This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only, The epitope for EB08557 is aa358-372.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 5 μg/mL.

Western Blot: Approx 38 kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1 kDa according to NP_001073007.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour.at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Andere Bezeichnung: XBP1

Hintergrund: XBP1, TREB5, X-box binding protein 1 , XBP2, OTTHUMP00000028682, X-box-binding protein-1

Gen-ID: 7494, 22433

NCBI Accession: NP_001073007

Pathways: ER-Nucleus Signaling, Unfolded Protein Response